LSM780 マニュアル

LSM780(もっと)簡易マニュアル[Z-Stack] 基本操作は2D編をご覧下さい。以下は画像調整が終わって画像取得ができる状態からの説明です。Z-Stackはフォーカスを変えながら連続切片像を取得する機能です。<Z-Stackの設定 しては英文マニュアルをご参照ください。 機器を有効にお使いいただくために、トレーナーによる取扱い説明を必ず受けるようにしてく ださい。不明な点がございましたら弊社担当までご連絡下さい。 取扱い注意 1 1. システムの起動. Carl Zeiss ipjTNM:wL:wTUM=wbk=-1-ZEN2010 B2AîBfB·B B B\B_Bt$ -~ R v PB B·BqBtBhB B®5ü ø3Ü ¾!ªB1 Ù îBxB B BaBbB]B*B Aï ZEN B*B2Aî &$³B B·B B.BKBN!¹ Ý çB BLAî }AñB-!¹ Ý £! AîB B·Bz. B (»B*B Aï Ä ` 6B*B21b ñBKB B öB 5ÂB Carl Zeiss ipjTNM L TUM=wbk= - 2- ※Safety lock 1. Starting the System 1-1. Starting the System 1-1-1. Switching on the LSM system 1-1-2. Switching on the X-Cite 120 or the HBO 100 mercury lamp 1-1-3. Switching on the A

3 START ZEN SOFTWARE 1. Start the software by clicking on ZEN 2012 icon on window desktop. 2. Click on the START SYSTEM button to have access to the acquisition menu. 3. Zen software is divided in 3 parts: the lef Make sure to use the 80/20 MBS along with checking the Reflection box in the light path menu. On LSM 780 and Conforcor 3 systems, make sure to use the standard PMT in Ch 1. Change the scan mode to line with a scan speed of 9 Close the pinhole to 1 AU to help reduce reflected light Carl Zeiss MicroImaging, Inc. Application and Product Suppor

概要 LSM780は蛍光観察が可能な共焦点レーザー顕微鏡です。 搭載されたレーザー光とピンホールにより、観察試料に焦点のあった極めて薄い範囲のみを観察することができます。 任意の蛍光の取得の他、微分干渉像や、位相差像も同時に取得する事ができます

全てマニュアルで光路を作成する場合には、以下のように設定します。 ① レーザのアイコンをクリックし、開いたウインドウから目的の蛍光色素を励起す るレーザを選択し、任意でレーザパワ ーを設定します。 レーザパワーは、2%程度か ZEN - シングルユーザインタフェースで、ZEISS光学顕微鏡イメージングシステムの全製品に搭載。 シンプルかつ素早い結果を出し、システム上で利用可能なオプションや適切な作業手順を導きます。 ZENは作業時間の節約、トレーニングやサポートの費用の軽減など、より早く結果を出すことを.

LSM780は高感度ディテクタ搭載で、今までより弱い光で明るい画像取得が可能です。32chアレイディテクタを搭載しているため、従来よりも高精度かつリアルタイムで分光ができます。搭載レーザーはDiode 405nm、DPSS 561nm、HeN LSM 800 with Airyscan - コンパクトな共焦点レーザスキャン顕微鏡 - 最高のイメージングクォリティ、高感度GaAsP検出器、高速linearスキャンによるフレキシブルな共焦点レーザスキャン顕微鏡。Airyscanにより、すべての次元で1.7倍の高分解.

③共焦点レーザースキャン顕微鏡LSM780 ④制御装置 ⑤防振テーブル 電源 ・ 三相200V 30A ・ 100Vコンセント (他オプション用) 900 1400 600 1200 800 600 800 800 超高解像度光学顕微鏡システム 構成図(平面図)・構成内 機器予約カレンダー利用マニュアル(PDF 1 MB) *2019年5月更新 ユーザー登録は こちら ※ユーザー登録後にメールアドレスやパスワード、その他登録情報を変更する際は、一旦ログインし、画面上部の「Edit user information」で変更してください そのときは机上のマニュアルにしたがってジョイスティックの再起動を行ってください。(2020/6/15) Zeiss LSM780の予約は使用日の3日前からです(土日祝も含む)。それより前に予約を取ることは禁止です INFORMATION FOR BSL2 WORK ON THE LSM780 CORE CONFOCAL MICROSCOPE, which can be requested from MatiukaA@upstate.edu) to the BioSafety Manual. 2. Once IBC approval is completed, contact Arvvydas.

Labs that use the following microscopes: Zeiss LSM780 or Nikon-SDC for 25 or more hours per month, during OFF-HOURS will be charged $30/hour for all use of that microscope. Labs that use the Zeiss LSM780 or Nikon SDC, live cell image system 75 or more hours per month, during OFF-HOURS will be charged at $20/hour for usage This product is an improved version of a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with SYBR® Green I LSM 880 with AiryScan 第4回 機器分析基礎セミナー 信州大学基盤研究支援センター 機器分析支援部門 蛍光顕微鏡の原理と応用 演者:末永 佳代子氏 カールツァイスマイクロスコピー株式会社 日時:平成29年6月1日.

Zentrum für Molekulare Biologie der Universität Heidelberg. 名古屋大学ライブイメージングセンターにある3つの顕微鏡ユニットの紹介です。多光子励起や高速イメージングも可能なポイントスキャン型共焦点顕微鏡、レーザーダイセクションと光ピンセット、赤外レーザー誘起遺伝子発現操作システムの3ユニットです LSM 980 with Airyscan 2 is the ideal platform for your confocal 4D imaging, optimized for simultaneous spectral detection of multiple weak labels up to 900 nm emission with the highest light efficiency. Add Airyscan 2 with its Multiplex mode to get more imaging options for your experiments. Choose the perfect setup to gently image larger fields. Zeiss LSM780 設置 : 2011年7月 特徴 温調モジュール、CO 2 モジュール、Definite Focus(カルチャーディッシュのフォーカスを安定化させる装置)を搭載し、ライブセルイメージングを目的としたシステムです. The Zeiss LSM 780 Confocal is an inverted modular microscope that allows for 3D high resolution imaging of a large variety of samples. It is compatible with a variety of sample chambers, including: two to eight-well chamber slides. petri dishes. multi-well plates

Lsm780 - 秋田大学 大学院医学系研究科・医学

光の当て方の違いで、こんなに変わる! 「光源から網膜までが顕微鏡」。光源からサンプル、そして対物レンズまでの経路にも、大切な選択肢があります。それは「どのように光を当てるか」という選択。観察したいサンプルは、色が付いているのか透明なのか、細かい構造を持つのか持た. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new feature Title METAを利用した画像の取 講座 光の回折限界を超える蛍光顕微鏡法PALM による細胞内微小構造の観察 205 1. はじめに 蛍光顕微鏡は光学顕微鏡の一種であり,その解像度dはエ アリーディスクで回折限界を規定したレーリーの判断基準に 従う(図1)(式1)

Zen Zeis

LSM780 is a black Renault Clio 5-door Hatchback from 2011, currently in traffic. It has a petrol engine with 55 kW (75 hp) power and an 5-speed manual transmission. It accelerates from 0 to 100 km/h in 13.4 sec. The combined fue 特集:裾野が広がる共焦点顕微鏡 - 共焦点顕微鏡の概要. 1.はじめに. 光学顕微鏡の歴史は400年の長きにわたっており、1590年のヤンセン父子による発明に始まり、ロバート・フックによる細胞の発見(1665年)、コッホによる結核菌発見(1882年)など、人間.

ViewRNA Tissue 1Plex Signal Amplification Kit. TFA-QVT0200、TFA-QVT0201は販売中止になりました. 研究用. Invitrogen ViewRNA ISH Tissue 1 Plex Assay Kitは、パラフィン組織切片や凍結組織切片に対応した、単色(Fast Red)染色用の高感度in situ Hybridization 試薬です。. シグナル検出のための. Institute of Experimental Botany of the Czech Academy of. 1 2 The Olympus FLUOVIEW Series — Giving both individual and group users the right solution to match their research needs and budget. The Fluoview FV300/FV500 offers a choice of two systems to meet the needs of an individua LSM780)、透過型電子顕微鏡(日本電子JEM-1400+)、次世代DNAシー ケンサースポット点検(ライフテクノロジーズ PGM-400M)、細胞撮影 装置スポット点検保守(GEヘルスケア IN Cell Analyzer 2000/2200 PrevMaint)等の保

Tohoku University Official English Website - Zeiss LSM780

Whether you are interested in the dynamics of mitochondrial movement, fusion and fission or budding of the endoplasmic reticulum, Elyra 7 Lattice SIM² provides you with the necessary live cell compatibility at super-resolution. U2OS cell expressing Tomm20-mEmerald. Objective: Plan-Apochromat 63× / 1.4 Oil The Zeiss LSM 780 point scanning confocal is an advanced version of the 710 that includes a 34-channel high-sensitivity (GAsP) detector. The spectral detector allows for fast-switching 4-colors imaging and, in extreme cases where spectral overlap is a limiting factor, emission fingerprinting. Laser lines: 405, 458, 488, 514, 561 and 633 n 形態解析イメージング研究室 利用・施設案内 目次 利用者登録 支援内容 依頼標本作製 研究支援 謝辞について 主要設置機器一覧 詳しくは医学研究科大学院シラバス「研究施設の概要」をご覧下さい。下記ページからダウンロード可能です

LSM 800 with Airyscan ZEIS

  1. 2020.9.10 【 お知らせ 】レベル2-での共同利用 室ご利用について 【 Notice 】Regarding the use of shared rooms at Level 2- 2020.9.2 ≪オープンファシリティセンターバイオ部門より/From Open Facility Center≫ 【 不具合解消のお知らせ 】倒立共焦点顕微鏡 LSM780
  2. Lsm780, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 86/100, based on 1 PubMed citations
  3. Zeiss共焦点レーザー顕微鏡LSM780 1時間あたり 医学系と学内937円・PCのみ36円,学外1514円・PCのみ36円 管理者からの連絡事項 顕微鏡の概要に記載した以外,使用時間について, 予約時間はできるだけ使用時間に即
  4. DIC Imaging using Laser Scanning Microscopes (LSM) on Inverted Stands Differential Interference Contrast (DIC) imaging is a technique used to increase contrast in brightfield images. In confocal systems, DIC images can b
  5. Zeiss LSM780 confocal microscope Cuisinart electronic pressure cooker DOWNLOAD MATERIALS LIST References Buckingham, M. Gene regulatory networks and cell lineages that underlie the formation of skeletal muscle. . 114.
  6. 780 User Guide Overview • Starting up the system • Viewing your sample • Turning on the lasers • Setting up a configuration • Taking an image Starting up the system 1. Follow the directions for Powering up the 780, this is located o

LSM780はカールツァイスマイクロイメージング社のフラッグシップと言える共焦レーザ顕微鏡であり、抜群に高い検出感度と低ノイズを誇ります。検出器受光面にGaAsPを採用することで量子効率を最大2倍に高めるのと同時に、光路、電気回 Olympus CX31 Instructions Manual Download Instructions manual of Olympus CX31 Microscope for Free or View it Online on All-Guides.com Zeiss LSM780 Confocal Microscope. A more advanced confocal microscope when compared to the LSM700, with a few additional features: Excellent for live-cell imaging experiments due to fast scan mode. Can image up to 8 frames per second with 512 x 512 pixel resolution. Examples of experiments include; live cell virus particle tracking, membrane. 共焦点レーザー顕微鏡 ツァイス LSM780 1,200円/ 30分 共焦点レーザー顕微鏡 ツァイス LSM510 700円/ 30分 3次元画像処理装置 ツァイス IMARIS 300円/ 30分 オールインワン蛍光顕微鏡 キーエンス BZ-X700 500円/ 30分 ツァイス アキシオフォト 融. 共焦

Zeiss Lsm780, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 86/100, based on 1 PubMed citations Carl Zeiss lsm780 set up Lsm780 Set Up, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://ww

機器予約カレンダー利用マニュアル (Pdf 1 Mb) *2019年5月更

蔡司是全球范围内先锋的光学与光电行业科技集团。蔡司集团专注于开发、生产和销售测量技术、显微镜、医疗技术、眼镜片、摄影和电影镜头、双筒望远镜和半导体制造设备等 Introduction to Spectral Imaging and Linear Unmixing - Spectral imaging combined with linear unmixing is a highly useful technique that can be used in combination with other advanced imaging modalities to untangle fluorescence spectral overlap artifacts in cells and tissues labeled with synthetic fluorophores that would be otherwise difficult to separate

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Features News Documentation Download Run ImageJ in Browser! Plugins Developer Resources Mailing List Link Carl Zeiss lsm780 clsm instrument Lsm780 Clsm Instrument, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions an Compact FLIM and FCS Upgrade Kit for Laser Scanning Microscopes. FLIM, FRET, and FCS in one turn-key system. Compact, easy-to-use and maintenance-free kit, customized to all major LSMs in various configurations for unlimited flexibility. Highest sensitivity with up to 4 detection channels. Fluorescence lifetimes from < 100 ps up to µs

Inverted Zeiss LSM 780 multiphoton laser scanning confocal

  1. NIH Home Page. Scientific Resources. NIMH Home Page. NIMH Intramural Program. NIMH Intranet. NINDS Home Page. NINDS Intramural Program. NINDS Intranet. Support is available on the mailing list and on the image.sc forum
  2. Carl Zeiss lsm 780 inverted confocal microscope Z drive operation flat right Flat ergonomic control knob on the right side recommended for combinations with mechanical stage or object guides right https://www.bioz.co
  3. 台灣儀器行 - 台灣規模最大的儀器公司之一,專業代理世界知名.
  4. Images were taken with a Zeiss LSM780 confocal microscope and processed with Zeiss Zen software. Proximity ligation assay HeLa or THP-1 cells were fixed and permeabilized as mentioned above
  5. PicoQuant GmbH, specialist in single photon counting applications has extended the compatibility of its upgrade kit for laser scanning microscopes (LSMs).The kit that upgrades microscopes towards Fluorescence Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS), now also supports upgrades of the Zeiss LSM780 and the established Leica SP2 confocal laser scanning microscopes.

Tohoku University Official English Website - 顕微

九州大学 学術研究・産学官連携本部 機器共用促進支援室 〒819-0395 福岡市西区元岡744 (伊都地区)パブリック2号館 研究企画課内 電話:092-802-2115(内線:90-2115) shareaid airimaq.kyushu-u.ac.jp ※「 」は「@」 Zeiss laser scanning confocal microscope LSM710, LSM780 and Leica laser scanning microscope SP8 containing laser lines at 488, 561 and 633 nm were used to image immunostained fixed or live embryos. The 40X objectiv Epifluorescence or confocal microscopy were performed using a Leica CTR6500 or Axio Imager M2 or Carl Zeiss LSM 700 (ZEISS) or LSM780 (ZEISS) microscope and Zen software. The Fiji software was.

Specifications A Zeiss LSM780 UV-NLO confocal microscope, one of only a handful worldwide, is a core ANU facility, housed within the Centre for Advanced Microscopy. This instrument was purchased through an ARC LIEF grant and generous support from individuals and groups throughout RSB and CMBE. This instrument is available to all researchers LSM780-FCS LSM800-GaAsP LSM880-AiryscanFAST SDC-3i (Live) SDC-Leica (MicroInj) Multiphoton, SHG Light, Specialized High-content Imager Lattice Light-sheet OMX SR SIM TIRF, dSTORM Super-Res Lumicks C-Tra Stage configuration. The system is based around an Axio Examiner Z1 upright fixed stage microscope that is designed to have highly configurable sample holders with a lot of space under the objectives. The motorized stage is usually in place which is good for slides and small samples. The height of this stage can be crudely adjusted (with the. Brüker Micro-CT Training and Appointments. Phone: (302) 831-3403. jcaplan@udel.edu. Office: DBI 117B. Sylvain Le Marchand. Zeiss 780 Confocal and Brüker Micro-CT Training and Appointments. Phone: (302) 831-3441

LSM780 ELYRA PS.1 A New Dimension in Confocal Microscope <セミナー内容> LSM 780 - GaAsP spectral detector を利用したFCS, FCCS のご紹介 LSM 780 特徴 ・32Ch GaAsP (ガリウム砒素燐) PMT を世界で初めての採用 Leica Microsystems: Optics for Your Discoveries We have designed and produced superior optics for a wide variety of applications in research, industry, and medicine for more than 160 years. Today, the innovation power of our optic Imaging was done by laser scanning confocal microscopy (Carl Zeiss LSM700, LSM780 and Leica TCS SP8). Processing of samples was carried out in tissues from littermates under the same conditions. Processing of samples was carried out in tissues from littermates under the same conditions Retinal section images were acquired on a Zeiss LSM780 confocal microscope, on a 40x oil immersion objective. Analysis Electroporated and immunostained retinas were quantified as 20 μm thick retinal cryosections imaged via confocal microscopy

名古屋大学 ライブイメージングセンター 機器紹

  1. Images were taken on a LSM780 laser scanning microscope (Zeiss). For imaging of GFP and mCherry, fluorophores were excited with 488 nm and 561 nm laser lines, and emission detected at 493-556 nm and 597-636 n
  2. eral oil using a 40x C-Apochromat 1.2 N
  3. images were obtained on a Zeiss LSM780 confocal microscope and processed with ImageJ. For further de-tails, see Additional file 2: Supplementary methods. Generation of single-nucleus RNA-seq from postmortem human brain.
  4. The represented images were captured by confocal microscopy (Zeiss LSM780, Jena, Germany). To further quantitatively analyze the cellular uptake of GO, cells were treated with FITC-BSA-GO or FITC-BSA for 24 hr, and then were collected and washed in PBS 2 times
  5. Instruments. Biological Optical Microscopy Platform. BOMP provides access to a wide range of different fluorescence microscopy systems. The table below provides a list of these sorted by location, with access to instrument specification sheets, booking calendars. For pricing information, see here
  6. Lambda stacks were acquired in z-series using a Zeiss LSM780 confocal microscope equipped with 20× 1.4 NA and 63× 1.4NA objectives, a GaAsP spectral detector, and 405, 488, 555 and 647 lasers. All lambda stacks wer

LSM 980 with Airyscan 2 - Confocal Microscope with

  1. Confocal microscopes are often used for the visualization and analysis of subcellular structures and biomolecules, such as proteins, in fixed and live specimens. You can find out more about applications where confocal microscopy is used from Science Lab, the microscopy knowledge portal of Leica Microsystems
  2. The light microscope has long been used to document the localization of fluorescent molecules in cell biology research. With advances in digital cameras and the In quantitative fluorescence microscopy, we want to measure the.
  3. Zeiss LSM780, Nikon Spinning Disk, Zeiss AxioImager, BioAFM, Imaris Workstation If you are on our External home page see Schedulable Equipment and Resources at bottom of page. Publication Acknowledgement As wit
  4. ation. ApoTome.2 is a module with integrated grid structures. Insert the module into your microscope and leave AxioVision to do the rest for you. Taking account of the objective used, AxioVision automatically places the optimal grid into the microscope's beam path - no manual change is required

九州大学医学研究院 教育・研究支援センター 設置機器一

Study Initiation Key Points. 294 KB. Key Points include: Informed Consent, Continuing Review Submission to the IRB, Confidentiality and HIPAA Issues, Amendments to Approved Protocols, Adverse Event Reporting to the IRB, Protocol Deviation Reporting to the IRB,DSMB Submission to the IRB. Study Initiation Visit: Preventive Care for Your Study Site Microscope and imaging: Images were acquired using Zeiss LSM780 Confocal harboring 405-, 488-, 561-, and 633-nm lasers. Image analysis was done using ImageJ software (Schneider et al., 2012). Details of image acquisitio Axio Examiner/LSM780 Detectors: descanned 32 GaAsP + 2 PMT and transmitted light detector Lasers: Coherent Chameleon XR (720-980 nm) + 405 nm, 458 nm, 488 nm, 514 nm, 543 nm, 633 nm Okolab temperature & ga Both cells and worms ROS levels were measured using a Zeiss LSM780 and AxioExaminer at λ = 530 nm. JC1 staining Cells were treated with different conditions: 6 h with 20 mM lactate or with 150 μM H 2 O 2 for 30 min

スタンダードマニュアル倒立顕微鏡 トウリツ ケンビキョウ ZEISS Axio Vert A1/AxioCam MRm, 正立型ルーチン顕微鏡 セイリツ ガタ ケンビキョウ ZEISS Axio Scope/AxioCam MRc 医学部基礎研究棟439号室 組織化学実験室 ソシキ カガ Zeiss LSM780: Live Cell Imaging Facility - UT Southwestern, Dallas, Texas Equipment Zeiss LSM780 Upright Zeiss LSM780 Inverted Zeiss LSM880 inverted Zeiss LSM880 Airyscan Nikon CSU-W1 SoRa OMX SR Deltavision Zeiss AxioObserver Zeiss Axioscop Zeiss SteREO Discovery Workstations Zeiss LSM780 Upright (ND5.74 About us. Department of Pathology. Research. Facilities. The microscopy facility houses a number of microscopes with the primary aim of facilitating high-quality research. The facilities available aim to provide tools to investigate both live-cell and fixed samples in 2D, 3D and 4D. We are able to perform light microscopy on containment level 2. It has an optional environmental chamber for live cell imaging experiments, but is used mainly for intravital imaging. 4 NDD detectors for higher signal detection. It is located in the In Vivo Imaging unit. To receive training and access to the unit please contact: brf-imaging@crick.ac.uk. calm@crick.ac.uk

Zeiss LSM 780 Confocal Centre for Dynamic Imagin

  1. Abstract. Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture
  2. FRAP was performed on a confocal microscope (Meta LSM780; ZEISS) with a 63×, 1.4 NA objective, at 37 C and in DMEM gfp2 complemented with rutin and 10% FCS. The translation foci labeled by the scFv-sfGFP wer
  3. Images were acquired with an LSM780 confocal microscope and Zen software (Carl Zeiss, Thornwood, NY). Quantification was performed using ImageJ for tumor area calculation and blinded manual counting of CD3+ cells
  4. A fundamental property of infectious agents is their particulate nature: infectivity arises from independently-acting particles rather than as a result of collective action. Assemblies of the protein tau can exhibit seeding behaviour, potentially underlying the apparent spread of tau aggregation in many neurodegenerative diseases. Here we ask whether tau assemblies share with classical.
  5. Our Imaging Lab provides instrumentation, training and support for faculty and students in the acquisition and analysis of data using state of the art imaging equipment. Here researchers have access to equipment including.
  6. DNA was stained with DAPI and samples were mounted and observed with a Zeiss LSM780 confocal microscope (Zeiss). Reverse transcription and real-time PCR Total RNA from embryos was extracted using the Arcturus Picopure RNA isolation kit (Life Technologies, Grand Island, NY, USA). cDNA synthesis was performed using a reverse transcription system (Invitrogen)
  7. Zeiss Upright Models Review Axiostar Plus - A smaller but high performance microscope that delivers robust features and upgradeability as well as the Zeiss renowned ergonomics. At a very nice price! Axio Scope A1 - This modular member of the Zeiss microscopes family was engineered for teaching at universities, research labs, industrial, and medical applications and used in quality control in a.

Mitochondrial DNA molecules coated with proteins form compact particles called mitochondrial nucleoids. They are redistributed within mitochondrial network undergoing morphological changes. The straightforward technique to characterize nucleoids' motions is fluorescence microscopy. Mitochondrial nucleoids are commonly labelled with fluorescent protein tags, which is not always feasible and. taken using the confocal microscope (LSM780, Carl Zeiss, Jena, Germany). The spreading area of each cell was calculated using ImageJ software. S-4 Supporting Table and Figures: Table S1. Cell surface marker phenotypes of. zeiss lsm780 操作手册 资源大小: 45.62MB 上传时间: 2018-09-13 上传者: ichou92 从Zeiss CZI图像中提取和显示ROI:两个程序可从Zeiss CZI图像中提取和显示ROI。-matlab开发 资源大小: 4KB 上传时间: 2021-05-28. Images were collected with a LSM780 laser scanning confocal microscope. Examples of EB1-GFP comets at the ends of astral microtubules are indicated with arrowheads. Scale bar: 5 μ m. (b) An example of a lattice light-shee

Live (green) and dead (red) cells with nuclei (blue) were imaged by confocal microscopy (LSM780; Zeiss, Oberkochen). Ex vivo aortic ring culture assay A rat thoracic aorta was harvested and rapidly dissected into 1-mm-thick rings in PBS with penicillin-streptomycin (1%, v/v; Gibco), followed by removing the surrounding fat and perivascular tissues and washing out the blood in PBS The course investment fee is: £1200 Academic Rate | £1600 Industrial Rate. As the number of places is limited to permit full hands-on practice, early registration is advised. To register for the Confocal Microscopy Hands-on Course, please fill out Registration form Confocal - Sept 2021 (MS Word , 25kb) and send to btf-enquiries@york.ac.uk

Zeiss LSM780 - retired 2021 - Turku Bioscienc

Islet inflammation is an important etiopathology of type 2 diabetes; however, the underlying mechanisms are not well defined. Using complementary experimental models, we discovered RIPK3-dependent IL1B induction in β cells as an instigator of islet inflammation. In cultured β cells, ER stress activated RIPK3, leading to NF-kB-mediated proinflammatory gene expression. In a zebrafish muscle. Excess dye was removed by a short wash with 50 mM Tris (pH 7) buffer, and then samples were mounted on slides and observed using a laser scanning confocal microscope LSM780 (Carl Zeiss) equipped with a plan-Apo × 63/ Cells were then incubated in darkness at room temperature (20-25 C) for 10-16 h, after which we observed GFP fluorescence using confocal laser microscopy (LSM780, Carl Zeiss, Jena, Germany). The OsHAK21 and OsAKT1 coding sequences (excluding their stop codons) were fused with EGFP and mCherry , respectively, at the C-terminal in the pSuper1300 vector